UCSI University Bioinformatics Assignment: Sequence Analysis Report

This bioinformatics assignment report details a step-by-step sequence analysis process. The report begins by identifying a gene sequence and determining its domains. BLASTn and protein BLAST searches are then performed to align the sequences. The report also includes the generation and analysis of a phylogenetic tree using a tree viewer tool and Clustal W for multiple sequence alignment. The assignment aims to apply computational methodologies to analyze biological datasets, specifically focusing on the practical application of bioinformatics tools such as BLAST and phylogenetic analysis. The report includes the results of the analysis and provides explanations of the findings, as well as the tools required for the analysis. This assignment is a part of the bioinformatics course at UCSI University.

Running head: BIOINFORMATICS
STEP BY STEP ANALYSIS
Name of the Student
Name of the University
Author Note

1BIOINFORMATICS
Table of Contents
Procedure....................................................................................................................................2
Tools required............................................................................................................................3

2BIOINFORMATICS
Procedure
The step by step analysis of the gene sequences goes as follows:
1. The name of the gene sequence is noted down first. This name is then searched for in
NCBI database or ENSEMBL database after selecting gene and nucleotide as the database
search class.
2. From the list of search results, the selected gene is chosen. After opening the sequence, the
domains are collected after scrolling down to the bottom of the page. These domains are then
written in the results and discussion of the report.
3. Then, BLASTn is searched for in Google which again leads to NCBI blast. Nucleotide
BLAST (BLASTn) is selected.
4. The query sequence is pasted there in FASTA format and the search is run. A list of
homology alignment results is seen on the next page. Out of these results, five best
alignments are used for the results and discussion.
5. Then protein BLAST is performed to get the protein sequence alignment in the same way
as stated for the nucleotide BLAST in step 4. All the search results are saved as an image and
explained in the observation section.
6. After generating the BLAST result, a phylogenetic tree is created and viewed with a tree
viewer tool. Multiple sequence alignment is required for this procedure.

3BIOINFORMATICS
Tools required
1. BLAST (online)
2. Tree Viewer (Offline)
3. Clustal W (Online)