Biology Assignment: Analysis of Mallomonas splendens gene sequence

This Biology assignment provides a detailed analysis of a gene sequence from Mallomonas splendens. The assignment begins with the identification of the type of BLAST search used, followed by identifying the organism and its subgroup. The solution then delves into specifics such as the number of nucleotides in the coding sequence, the STOP codon, and the function of the untranslated sequences. Further, it explores the number of amino acids encoded by the gene and the reason behind the relationship between the number of nucleotides and amino acids. The assignment concludes with a description of the encoded protein, its location, and the types of cells that utilize it. References are also included to support the findings. This assignment provides a comprehensive understanding of the gene's function and its role within the organism.

Biology 1
BIOLOGY
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Instructor
Institution
Location
Date

Biology 2
Question 1
The type of BLAST search chosen for this BLAST was nucleotide blast. The DNA sequence
provided for the purpose s of this assignment was of a nucleotide sequence.
Question 2
Name of the gene: splendens
Question 3
a) Organism from which the unknown sequence has been derived
Mallomonas splendens
b) Subgroup of the organism
Phylum Ochrophyta
Question 4
Full reference of the publication
Beech, P.L..S..V..G.R.a.M.I., 1999. Direct Submission. Sydney: School of Botany, University of
Melbourne.
Question 5
Number of nuclides in the coding sequence for this protein
498
Question 6
The STOP codon for the gene is TGA (Beech, 1999, p.1230)
Question 7
What we call the two sets of sequences that are downstream of the coding sequence in this
transcript of the gene in the question

Biology 3
Flanking and untranslated sequences
Question 8
State one function of one of the sets of sequences from Q7
Untranslated sequence-Forms the site of initiation of transcription
Question 9
How many amino acids are present in the entire protein that the unknown sequence encodes part
of?
Three nucleotides encode one amino acid in the sequence. Due to the presence of 64
combinations of 4 nucleotides which are taken three at a time, 20 amino acids will be produced
by the entire protein sequence (Association, 1992, p.897).
Question 10
Why the number of nucleotides that encode the protein is not exactly 3 times the number of
amino acids that are translated
Encoding of amino acid requires a minimum of three nucleotides, which are referred to as a
codon. A single codon encodes just one amino acid at a time hence three nucleotides are required
to encode one amino acid (Ohno, 2013, p.569).
Question 11
Description of the encoded protein

Biology 4
The unknown sequence of protein is for Mallomonas splendens Ftz mRNA. It is a nuclear gene
that encodes a protein of the mitochondria, complete cds (Biology, 1993, p.158). The unknown
protein is thus found in the mitochondria organelles of the organism. Among the organisms that
have this protein sequence include Eukaryota, Stramenopiles, Synurophyceae, Synurales,
Mallomonadaceae and Mallomonas species of organisms. The protein being found in the
mitochondria of the organism is thus fundamental in the facilitation of the processes of
respiration in the organism. The types of cells that use this protein are muscular cells, respiratory
cells and other cells involved with processes that are associated with the breakdown of glucose in
the body to release energy (Beech, 1999, p.256).

Biology 5
References
Association, A.M., 1992. Journal of the American Medical Association, Volume 267, Issue 9.
Journal of the American Medical Association, Volume 267, Issue 9, 267(9), p.1450.
Beech, P.L..S..V..G.R.a.M.I., 1999. Direct Submission. Sydney: School of Botany, University of
Melbourne.
Biology, A.S.f.C., 1993. The Journal of Cell Biology. The Journal of Cell Biology, Volume 2,
p.1711.
Ohno, S., 2013. Evolution by Gene Duplication. Evolution by Gene Duplication, 226(7), p.160.