University Assignment: Cellular Organelle Isolation Assessment Report

This report assesses the isolation of cellular organelles, focusing on the methods used to determine their purity and functionality. It details the use of a Clarke-type oxygen electrode for measuring mitochondrial oxygen consumption, linking it to ATP production, and discusses enzyme activity assays to assess the purity of the isolated organelles. The report also covers the application of SDS-PAGE for protein analysis, highlighting its role in determining molecular weight and complex protein formation. Furthermore, it outlines a strategy for isolating the Golgi apparatus from wheat, emphasizing the importance of homogenization, differential centrifugation, and proteomic analysis, including the use of western blot analysis and affinity purification methods to ensure purity. The report also describes the assessment of Golgi apparatus purity using western blot analysis with specific markers and the biotin switch assay for S-nitrosylation analysis.