Clinical Trial Report: Evaluation of Consumer Perception in Skin Care

This clinical report presents the findings of a consumer perception trial comparing topical L-Ascorbic acid (Vitamin C) and Retinol (Vitamin A) for skin rejuvenation. The study involved a non-randomized group of 63 participants (62 female, 1 male) aged 18-50, who applied Vitamin C to one side of their face and Vitamin A to the other, twice daily for six weeks, along with sunscreen. The report details the methods, including quantitative data collection on various perception criteria such as product texture, pigmentation, and effects on fine lines and skin texture. Results are presented, including participant ratings and comments on both substances, noting the side effects. The report evaluates the perception of each product, highlighting that while some found L-Ascorbic acid too heavy, the majority found Retinol more comfortable on the skin. The discussion section evaluates the observed results, clinical trials of Ascorbic acid and Ratinol, the side effects and comments which were delivered by some participants about the product.

CLINICAL TRIALS

Table of Contents
ABSTRACT.....................................................................................................................................3
INTRODUCTION...........................................................................................................................4
METHOD........................................................................................................................................5
RESULTS........................................................................................................................................5
DISCUSSION..................................................................................................................................9
REFERENCES..............................................................................................................................11

ABSTRACT
Background
Ascorbic acid is also called as vitamin c it is defined as the vitamin found in radius foods
and sold as a dietary supplement. As per this, the retinol is also used to sold in the market as per
best known for skin care in ingredient.
Objective
Moreover, the consumer perception is usually analysed for this element such as ascorbic
acid and retinol are usually have a cold tendency in order to enhance the attractiveness and
influence the audible situation for others. Moreover, usually consistent with that they used to
provide and attractive recognition due to the changes in the melanocyte factors moreover this
report also states about various factors which is associated with ascorbic acid and usually in the
psychological views on this is to provide the energy and outsource the formation of adenosine
triphosphate in order to provide better health and rational for this.
Methods
However, the retinal is usually identified as a skin care equipment and order to change the
viability of fairness and create resistivity from the deserts and foreign particles. In this, all data
are usually collected by quantitative approach which make a stratified and cluster sampling
framework usually it is a core concept of the perception of the consumer which arises as per the
buying such kind of product which contain ascorbic acid and retinol. While, there is a various
consequences and factors are usually associated with the over excessive use of ascorbic acid and
retinol. Moreover, the ascorbic acid can induce more and more nutrition and vitamins in the body
and can create the complex situations due to the breakdown clusters of the vitamins in the body.
Overall, the excessive retinol use can create skin irritation and dermatological problems related
with the individual.

INTRODUCTION
In human, there are some of the visible science resource is going in skin. These are like
wrinkle, rough, itchy and dry skin which involves loss of the elastic tissue within a skin,
transparent and non cancerous tumors. Intrinsic aging genetically determined to age. This is
generally in genes. This is a natural and generally affected by body hormones level which is
therefore in within cells and protein. Intrinsic aging refers to an inevitable physiological process
which creates dry and thin skin. Gradual dermal atrophy find wrinkles and white extension aging
created by external environmental factors like smoking, pollution and sun exposure. Extensive
aging refers to a result of environmental factor and different lifestyle choices which occurs to the
skin (Pontoriero, (2019).This creates look of skin more years older than actually age. This is due
to poor maintenance like not drinking enough water, skipping sunscreen but it is easier for
preventing by extensive caring and repairing of skin.
Antioxidants refers to the substance which are used for preventing and slowing damage
of cells which was caused due to an unstable molecule of free radicals which are produced within
body as a environmental reaction and other factors. Free radicals producing molecules during
breakdown of food to provide skin and can have better ageing. Vitamin C is also known as well
as Ascorbic acid which refers to a water soluble vitamin which are generally present in foods and
dietary supplements. Human are not able to produce Vitamin C endogenously. So, Vitamin C is
essential dietary component. Vitamin C are generally involved in protein metabolism and play an
important physiological antioxidant role for regenerate other antioxidants made in body. This
includes vitamin E (Gao, (2017). When there is topical treatment with using Ascorbic acid, there
is elevation of skin inflammation which is generally mediated by species of reactive oxygen in
UV radiation and human skin. It also helps to provide a protective effect on the inflammation
due to ultraviolet sunburn. Ascorbic acid refers to a salt which are generally used and cosmetic
formulation and personal care as an antioxidants which can help to create a slow deterioration of
skin due to sunburn. Retinol also known as the vitamin A generally found in food and used as
dietary supplement. This fulfill the deficiency of vitamin A. In normal dose of retinal can be
tolerated but higher dose of it, can create a dry skin enlarged liver or Hypervitaminosis A.
During pregnancy high dose can create a negative impact on baby. There is medical use of
Retinol which is used in to treat deficiency of vitamin A. Retinoids are helpful in reducing fine
lines and wrinkles by enhancing the production of collagen. This helps to produce new blood

vessels within skin which helps in improving skin colour. Retinol helps the skin to get a more
prone to sunburn. Retinol can help to increase skin cell turnover and able to stimulate the
collagen production within body. This helps to provide improvement that create a better
appearance of fine lines and wrinkles which helps to improve skin texture and show flawless
looking skin (Ojeda, (2019).
METHOD
As per the method is quantitative in nature and this is analysed by various searching of the
perception criteria and rating as per the reported of participants. Moreover, the ascorbic acid is
usually identified as various services which include the product texture and consistency which is
comfortable by number of populations effect on pigmentation the rating is slight improvement by
number of population effect on the fine lines the rating is no changes which is approved by 41
population and many more factors which are rated by the taking number of populations
(Arayachukiat (2017). Moreover, there are various texture improve softness are usually analysed
by 4 participants whereas 4 participants state that there is unpleasant sticky applies an evenly. In
this, the product consistency is very light because the individual with a dry skin may need to use
a heavy moisturizer in order to compensate. Moreover, the 62 light and dry skins are observed
where three participants are used to review as inappropriate for oily skin types and the
improvement is needed and providing feedback from the three participants. In this, it shows the
side effect of arrhythmia and rated it up to because it has a limited and shorted for the significant
improvement.
Method 1 refer to Annexure B (Materials and Method) – The study was conducted to compare
topical Vitamin C and vehicle for rejuvenation where 10 participants having facial photodamage
were recruited, etc.
Method 2 refer to collated results- A consumer perception trial was conducted for 6 weeks to
compare topical L Ascorbic acid also known as Vitamin A and Retinol also known as Vitamin
C . The trial included a non-randomised group of 63 participants of which 62 participants were
female and 1 male with the age group ranging from 18–50-year-olds. Vitamin C was applied to
the right side of the face and vitamin A was applied to the left side of the face twice a day for 6
weeks. Participants were also encouraged to apply broadspectrum sunscreen.

RESULTS
Ascorbic acid:
PERCEPTION RATING NUMBER OF
PARTICIPANTS
Product texture and their
consistency on skin
Heavy
Easy to use
Light
3
32 +1
27
Pigmentation Change is occurred
Need improvement
Proper improvement
20 +1
39
3
Effects of line No change needed
Need improvement
Proper improvement
41 +1
20
1
Effect on skin content No change needed
Need improvement
Proper improvement
22
38 +1
2
Surface skin and their texture No change needed
Need improvement
Proper improvement
16
38 +1
8
Side effect and their initial
application
Low
Moderate
High
35 +1
24
3
Side effect Low
Moderate
High
56 +1
4
2
Other side effect:
3 participants are used report in acne lesion
1 participant state about the mild erythema.
3 participants reported about the enhancement in the skin dryness and need moisture after use.
3 yellowing of finger in hand.

1 congestion
1 oxidation and have sense of the blocked pores.
Comments:
Product consistency is very light, individuals with dry skin may need to use a heavy moisturiser
to compensate. 6 too light, dry skin 4 skin texture improved, softened 3 inappropriate for oily
skin type 4 unpleasant sticky, applies unevenly 3 improvement in PIHP 4 unpleasant odour
Interpretation:
16 out of 36 participants reported that Retinol reduced pigmentation, then 44% is notified
(Divide 16 by 36 and then multiply by a hundred to convert to a percentage).
Retinol:
PERCEPTION RATING NUMBER OF
PARTICIPANT
Product texture and
consistency
Heavy
Easy
Light
4
49 +1
9
Pigmentation No change
Need improvement
Proper improvement
47 +1
15
0
Effect on fine line No change
Need improvement
Proper improvement
42 +1
17
3
Effect on the surface of
texture
No change
Need improvement
Proper improvement
39
12 +1
11
Side effect for the initial
application
No change
Need improvement
Proper improvement
12
39 +1
11
Moisture content No change 51 +1

Need improvement
Proper improvement
11
0
Side effect: erythema Low
Moderate
High
45 +1
10
7
Other side effect:
In this, some of the additional reports are advised in order to gain more side effect which is
caused by the factor and use of retinol. These are analysed by the participant which is used this
product (Mendu and et. al., (2019).
2 participants report about the dryness and irritation
1 participant note that skin was red and felt the nature warm.
Stopped it excessive use due the pimple.
Comments:
After analysing the feedback comments from the participants which is usual and provided with
the relevant. In this, three participants are used to comment that they have not sufficient
hydration in order to achieve the moisture. Moreover, one participant is used to reduce the acne
lesions which is present inside the retinol. Whereas, two texture and smells are commented. In
this, 7 are used to enjoy the product and make an approach toward the product while saying that
the product is well nourished and used to when hydrated the body and the skins for proper
period. Moreover, the 5 participants are used to comment it in order to improve the lines and one
is committed as a to oily and two are comment is are the two stickies. Moreover, as per all the
one stopped treatment due to the reaction in which the reaction is not reported by the participants
(Pantwalawalkar and et. al., (2021).
Interpretation:
Each perception criteria needs to explained for example : to change into % - divide 3 by 63 and
then multiply by 100 = 4.76% round it to 5% . So instead of 3 in the table – it should be 5% in
the table.
As per the percerption criteria – product texture and consistency, 5% participants found L-
Ascorbic acid to be too heavy and 6% complained the same for Retinol, 52% participants found
L-Ascorbic Acid comfortable, whereas 79% of participants found Retinol more comfortable on

the skin. When applied to the skin, 43% participants found L-Ascorbic Acid to be too light and
only 14% of participants found Retinol to be too light (Díaz, (2020).
DISCUSSION
As per the observed result is can be evaluate that there are different clinical trails of Ascorbic
acid and Ratinol. There are various trial of Ascorbic acid. It has been observed that there are
comfortable texture and consistency within the product. There is no effect of pigmentation has
been observed by participants. There is also taken off criteria for effect on fine lines in which no
change were observed by 42 participants from 63. Skin surface texture trial was need of slight
improvement which was recommended by 39 participants and other trial was normal and
recommended no need for change. It has been observed that there are some of the side effect
which was observed by different participant about different issues like increase in acne,
increased dryness, oxidation, yellowing of fingers (Martí, (2018). There are some of the
comments which were delivered by some participants about the product where participants
advice that product is not appropriate for oily skin and having unpleasant order along with
thickness which creates negative impact of product and needs to be improved for better use.
There are different perception criteria has been used for evaluating the Retinol by different
participants where product consistency and texture was comfortable according to 49 participants
and there should no any change on the effect of pigmentation which was according to highest
number of participants. There are many participants who advise for surface texture which
includes roughness and should be improved about the side effect arrhythmia. Initial application is
skin moisture content should have no need to change which can provide better impact during
using product. There were two participant who reported irritation and dryness whereas 1
participant feel that there is skin get red and warm. Through the result, there is need for normal
improvement which can provide sufficient hydration and need to reduction in thickness which
should not create issue for any consumer. There are some confounding variable which can be
improved by focusing on a strange texture and smell and hydration sufficiency which can created
improvement in the product. There is need to add one more clinical trial which is related to
change in body temperature after using or consuming the Ascorbic acid or retinol. It should not
able to change body temperature. When participates provides the feedback of no negative impact
and no any change in body temperature are observed. This provides better impact and result
which can provide improved efficacy of the product and should have a better and improved

result on human. From result, it has be evaluated that there is need to more trail on other group of
participants for better result and diverse feedback which should provide more diversity of
opinion and opportunity for more improvement in product. There is also need to take other group
feedback which can able to provide better result and interpretation of more population who can
provide better data regarding efficacy of product. This is able to provide better interpretation of
result for further procedure.

REFERENCES
Books and Journals
Martí, (2018). Polyphenol and L-ascorbic acid content in tomato as influenced by high lycopene
genotypes and organic farming at different environments. Food chemistry, 239, pp.148-
156.
Díaz, (2020). l-Ascorbic acid alkyl esters action on stratum corneum model membranes: An
insight into the mechanism for enhanced skin permeation. Colloids and Surfaces B:
Biointerfaces, 185, p.110621.
Arayachukiat (2017). Ascorbic acid as a bifunctional hydrogen bond donor for the synthesis of
cyclic carbonates from CO2 under ambient conditions. ACS Sustainable Chemistry &
Engineering, 5(8), pp.6392-6397.
Ojeda, (2019). Chitosan/tripolyphosphate nanoaggregates enhance the antibrowning effect of
ascorbic acid on mushroom slices. Postharvest Biology and Technology, 156, p.110934.
Gao, (2017). Facile synthesis of enzyme functional metal-organic framework for colorimetric
detecting H2O2 and ascorbic acid. Chinese Chemical Letters, 28(5), pp.1006-1012.
Pontoriero, (2019). Natural Antimutagens. Chemopreventive Action of L-Ascorbic Acid and
Green Tea Infusions on the Acute Toxicity and Mutagenicity of Reaction Mixtures
Nitrite-Sulfonamide. Cancer Therapy, 3, p.46.