Genetics Lab Report: DNA Extraction, Analysis, and Identification

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Added on  2022/07/11

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This report details a genetics experiment focused on the extraction and analysis of genomic DNA from a given sample, identified as white shark tissue. The experiment involved tissue cleaning, DNA sequence identification using dideoxy sequencing, and quality/quantity analysis. The methodology included cell lysis, DNA purification, and the use of various solutions. The report explains the principles of DNA, Chargaff's rules, and the methods employed, such as agarose gel electrophoresis and spectrophotometry for determining DNA purity and concentration. The procedures, including the use of a Scientific NanoDrop Spectrophotometer, are described in detail. The report also covers the materials and methods used in the experiment, including the preparation of buffer solutions, tissue processing, and DNA precipitation and washing steps. The analysis of DNA using both gel electrophoresis and UV spectroscopic methods is discussed, providing insights into the experimental process and results.
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