Presentation: Standard Plate Count & IMViC Profile Test - E. Coli

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Added on  2023/04/06

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This presentation showcases the results and discussion of a microbiology experiment involving standard plate count and IMViC profile tests on minced chicken samples. The standard plate count revealed a high number of colonies in both fresh and abused samples, indicating potential contamination. The IMViC profile test showed positive results for Indole and Methyl Red, and negative results for Voges-Proskauer and Citrate (with one potentially contaminated Citrate test). Based on these results, the presence of E. coli in the abused chicken sample was confirmed. The presentation references relevant studies to support the findings and emphasizes the importance of these tests in assessing bacterial contamination.
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Module 1
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Methods
Standard Plate count IMViC profile test
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Results
Standard plate count
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Results (Contd.)
IMVIC profile test
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Discussion
More than 300 colonies have been found in standard plate count for all the serial
dilutions ranging from 10-1 to 10-4.
Higher number of colonies denotes higher chance of contamination infection
(Davis, 2014).
Positive Indole test (presence of pinkish red layer) signifies the presence of Indole
test positive bacteria (Pant, Nirwal & Rai, 2013).
Positive Methyl red test also signifies the presence of bacterial contamination
(Odonkor & Ampofo, 2013).
Voges- Proskauer test and Citrate test both are negative for this investigation.
From the above IMViC profile, it can be confirmed the presence of
E. Coli in the
abused chicken sample (Mainali et al., 2013).
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References
Davis, C. (2014). Enumeration of probiotic strains: review of culture-dependent and
alternative techniques to quantify viable bacteria.
Journal of Microbiological
Methods,
103, 9-17.
Mainali, C., McFALL, M. A. R. G. A. R. E. T., King, R., & Irwin, R. (2013). Evaluation
of antimicrobial resistance profiles of Escherichia coli isolates of broiler chickens at
slaughter in Alberta, Canada.
Journal of food protection,
76(12), 2045-2051.
Odonkor, S. T., & Ampofo, J. K. (2013). Escherichia coli as an indicator of
bacteriological quality of water: an overview.
Microbiology research,
4(1), e2-
e2.
Pant, R., Nirwal, S., & Rai, N. (2013). Prevalence of antibiotic resistant bacteria and
analysis of microbial quality of raw milk samples collected from different regions of
Dehradun.
International Journal of PharmTech Research,
5(2), 804-810.
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