Report: Electron Microscopy of Subcellular Structure and Applications

This report provides a comprehensive overview of electron microscopy (EM) as a powerful imaging technique used to study the ultrastructure of cells and molecules. It begins with an introduction to microscopy and the advantages of EM over light microscopy, highlighting the development of electromagnetic lenses and the evolution of specimen preparation techniques. The report then details the working principles of both Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM), including their respective uses of electron beams and electromagnetic lenses. It explores various biological applications of EM, such as in cell biology, protein localization, and lipid studies, including the use of techniques like cryosectioning and immunogold labeling. The report also discusses the limitations of EM, such as the low penetration power of electron beams and the potential for sample damage. Finally, it concludes by emphasizing the indispensable role of EM in modern biological research and the ongoing efforts to improve its applications.