Practical Report: Gene Amplification & Testing of HeLa Level Changes

This practical report details an experiment focused on gene amplification and the analysis of HeLa DNA levels using agarose gel electrophoresis and PCR techniques. The methodology involved four main PCR reactions: HeLa control, ATO-treated HeLa cells, and a no-template control. The results, visualized through gel electrophoresis, were used to investigate the effect of ATO on the HPV E6 gene, with lanes representing different treatment conditions. The discussion section analyzes the gel electrophoresis results, including the determination of DNA fragment sizes and the identification of potential experimental errors. The report highlights the importance of DNA ladder standards and discusses factors affecting band clarity, such as DNA concentration and sample purity. The conclusion summarizes the experiment's aim, which was to amplify HPV E6 genes and examine changes in HeLa DNA levels, while acknowledging potential sources of error and the overall adherence to experimental standards.