This report provides an in-depth analysis of evidence-based practice concerning hand hygiene in healthcare settings. It begins with an introduction to Evidence-Based Practice (EBP) and its importance in improving healthcare quality, specifically focusing on the role of hand hygiene in preventing cross-infections. The report then reviews the literature, summarizing several studies that explore interventions to enhance hand hygiene compliance among healthcare professionals, including nurses. It highlights the importance of facility design, education, unit-level protocols, and multimodal interventions. The results section presents the findings of these studies, categorizing interventions and analyzing their effectiveness across different healthcare settings. The discussion section analyzes the findings, focusing on the impact of different interventions and the challenges in generalizing findings across various healthcare environments. The report concludes with recommendations for future research and practice, emphasizing the need for ongoing efforts to improve hand hygiene compliance and patient safety. The report also uses the World Health Organisation (WHO) guidelines and other research papers to support its claims and findings. This report is a comprehensive overview of the current state of hand hygiene practice.