Microorganism Isolation, Cultivation and Counting Methods Report

This microbial laboratory report details the isolation, cultivation, and counting of microorganisms, focusing on techniques such as pour plating, surface spread plating, and the most probable number (MPN) method. The experiment involved preparing serial dilutions of E. coli and utilizing these dilutions in various plating methods to determine colony-forming units (CFU). The report also covers direct counting methods using a haemocytometer and turbidimetric methods using a spectrophotometer to estimate viable cell concentrations of Saccharomyces cerevisiae. The procedures included the preparation of dilute series, surface spread method, pour plate method, MPN technique, direct microscopic counts, and spectrophotometry. The report discusses the principles, procedures, materials, and limitations of each method, providing a comprehensive overview of microbial analysis techniques.