This project delves into the performance analysis of Compression Ignition (CI) engines utilizing biodiesel as an alternative fuel source. The study highlights biodiesel's potential as a biodegradable and renewable energy source, addressing the increasing concerns of fossil fuel depletion and harmful greenhouse gas emissions. The research emphasizes the importance of biodiesel due to its calorific value, which is comparable to diesel, and its environmentally friendly nature. The analysis covers the advantages of biodiesel, such as its higher ignition temperature, excellent lubricity, and compatibility with existing diesel engines. The project discusses the significance of biodiesel in the context of global efforts toward energy security and climate change, advocating for its adoption as a key component of sustainable energy strategies. Furthermore, the project acknowledges the growing demand for alternative fuels and the need for stricter emission standards, underscoring biodiesel's bright future due to its biodegradable nature and reduced exhaust emissions.