Analysis of Unknown Drug D's Mechanism on Rat and Guinea Pig Ileum

This practical assignment investigates the pharmacological effects of an unknown drug, designated as Drug D, on both rat and guinea pig ileum smooth muscle tissues. The study employs a range of agonists and antagonists, including atropine, mepyramine, histamine, acetylcholine, and adrenaline, to determine the drug's mechanism of action. Experimental design involves administering varying concentrations of Drug D and observing its contractile force and EC50 values. The effects of antagonists are assessed to identify receptor types involved. Results are presented graphically, comparing the contractile responses of the smooth muscle tissues under different conditions, such as the presence of antagonists. The discussion interprets the findings, relating them to known pharmacological principles and referencing relevant literature to explain the observed effects of the unknown drug and its interactions with other compounds. The study aims to understand how Drug D interacts with specific receptors and affects the physiological responses of the ileum smooth muscle.