Protein Purification: Comparative Analysis of Purification Techniques

This report delves into the critical process of protein purification, a vital research area, particularly for applications like albumin purification in critical care settings. It explores the challenges and methods involved, emphasizing the use of ion exchange chromatography, specifically comparing the efficacy of Carboxymethyl cellulose (CM-cellulose) and Diethylaminoethyl (DEAE) cellulose exchange resins. The report details the experimental procedures, including buffer preparation, pH adjustments, and the use of SDS-PAGE for analyzing protein purity and separation. The findings reveal that CM-cellulose exchange resin yielded superior results compared to DEAE cellulose. The report also addresses the determination of molecular weight and isoelectric points of unknown proteins using SDS-PAGE, providing a comprehensive overview of protein purification techniques and analysis.