Fluorescence Microscopy of Osteosarcoma Cells with Verteporfin

This report presents an investigation into the effects of verteporfin treatment on human osteosarcoma cells, utilizing fluorescence microscopy for detailed analysis. The study employed U2OS osteosarcoma cells, treating them with varying concentrations of verteporfin and subjecting them to infrared laser treatment for different durations. Fluorescence microscopy, using probes like Hoechst33342 (for nuclei), Phalloidin 488 (for actin cytoskeleton), and Zombie red (for plasma membrane permeability), was used to capture cellular images and assess integrated density, roundness, circularity, and nuclear counting. The experiment involved different filter sets (blue, green, and red) and image analysis was carried out using Fiji Image J software. The objective was to understand verteporfin's therapeutic potential by examining its impact on cellular components and cell death, providing insights into potential treatments for metastatic osteosarcoma. The report follows the structure of The Journal of Immunology, including an abstract, introduction, materials and methods, results, and discussion.