Effects of Antibiotics on Bacterial Growth

RUNNING HEAD: EFFECTS OF ANTIBIOTICS ON BACTERIAL GROWTH 1
Effects of Antibiotics on Bacterial growth
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EFFECTS OF ANTIBIOTICS ON BACTERIAL GROWTH 2
Effects of Antibiotics on Bacterial growth
AIM
The aim of this experiment is to investigate the effect of different antibiotics on the
growth of bacteria and the effect of different concentration of penicillin on the growth of
staphylococcus aureus.
INTRODUCTION
Antimicrobial drugs can basically be defined as those chemotherapeutic substances that
usually hinder the growth of different microorganisms that might be different in different tissues.
This substances differ from antiseptics as well as disinfectants in that they have a high
therapeutic index. This means that they have a high toxicity to the microorganism than the tissue
(Kong, Yu, Zhang, Li, & Wang, 2017). This antibiotics also possess a very distinct mode of
antimicrobial action. They can be introduced to the body either through oral, parenteral or topical
application.
Antibiotics are usually produced by different living organisms which kill or impair the
growth of other microorganisms and some are not toxic to be used like antimicrobial
drugs .Majority of the antimicrobial drugs that are utilized in both the human and veterinary
medicine are usually antibiotics that have a high therapeutic index.
Majority of the antibiotics will hinder the growth of an array of bacteria. These are often
referred to as broad spectrum antibiotics for example tetracycline. Other antibiotics are restricted
in nature and are known as antibacterial spectrum (Pál, Papp, & Lázár, 2015) An example is the
penicillin which is usually active against Gram Positive bacteria that include Neisseria and the

EFFECTS OF ANTIBIOTICS ON BACTERIAL GROWTH 3
spirochetes. Streptomycin on the other hand is most active against Gram negative and the acid
fast bacteria.
There have been important techniques that have been used of late to develop antibiotics
and chemotherapy. They include drug sensitivity tests which determine the drug that is effective
against a particular strain of bacteria and the streak tests that determines the antibacterial
spectrum (Urish et al., 2016).Finally the quantitative antibiotic assays which determines the
quantity of an antibiotic in solution.
Method.
Take 3 Nutrient agar plate label it with bacteria name.
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Take one molten agar tube from the water bath wipe it out with the tissue.
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Add 0.2 ml of bacterium culture in to the molten agar with aseptically between a flames.
And mix it well.
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Then pour that molten agar with culture on the given N. agar plate and cover it with that agar.
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For solidifying put this plate on a table for 10-15 mins.
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Take a force sterilize it with the help of ethanol and flame.
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Take Multidisc from the box with the help of this sterile forceps and put that disc on middle of
the agar.
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