Assignment on Electrophysiology
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Added on 2020-04-01
Assignment on Electrophysiology
Added on 2020-04-01
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21)The main aim of electrophysiology is to record the activity of neurons. In vivo methods are used to record the activities from a single neuron. Through this method, the neuron cells are able to pass the important message through integration and propagation of electrical signals. The neurons are of different sizes and shapes but they play the same organized function of the transmission of the signals. The dendrites are able to receive the signals from other neurons through this method and then send them through synapses. The major difference in this method and the extracellular recordings performed in lab is that this method is able to involve both chemical and electrical signals in the transmission of the signals (Gustavo et al., 2012). The laboratory test is able to involve only the electrical signals when transmitting the signal. The fact that the extracellular recordings is under control means that one can divert the signals to follow different paths and therefore able to record different recordings from multiple neurons. In addition, the.polarization of the membrane is able to allow the observation of the activity at different potentials and therefore able to record it at different points (Gordh and Headrick, 2009).The action potential is able to pass at one point and therefore causing a recording at that point. The extracellular electrodes are able to detect very small potential changes and therefore making it possible to record those changes. Lastly, the electrode is usually connected to the positive inputof the voltage recording. This makes it possible to record the action potential whenever they happen. 2)The neuronal property, which is able to account for the stereotyped shape of action potential, is the distance of placement of the electrodes from the neuron. The stabilization of the electrical conductivity is usually achieved when the electrodes are placed on far distance from the neurons
3(Gustavo et al., 2012). The stabilization of the acyional potential is able to produce the same stereotyped shape. Similar shaped will be produced at this level and able to lead to similar properties and shape type. When the electrodes are placed near the neuron, the action potential from the positive electrode and the negative potential from the negative electrode are able to collide. This is able to cause difference in the shape produced at any moment. Another key element, which is able to lead to the difference of the stereotyped shapes, is the extracellular fluid. The fluid is able to account for the speed at which the signal will be send and therefore the detection level. Ionic currents are able to move at different speed leafing to the.formation of the different shapes at the end (Della, Santinaa and Lewisa, 2013). The electrical potential measuringinstrument is at times able to result to the stereotyped shape. The manner on which this instrument is able to measure and record the potentials is able to dictate the shapes, which will beachieved at any given moment between two points. The instrument nature of sensing the signal will therefore be a key property on the shape, which will be produced. The electrical potential between the electrodes will determine the speed and recording of the shape at the different points. 3)The filtering of the voltages is able to range from 0.1Hz and 300 Hz on the high pass and on the low pass filters between 0.3 to 20 kHz coupled with 60 Hz notch filters. Signals have to be filtered and observed properly at different frequencies in order to allow the amplification of action potential. Filtering of the voltages is essential to ensure that the best response is attained atspecific voltage during the filtering process. The action of the cockroach's nerve action will differ according to the voltage at which it is exposed unto (Kruszelnicki, 2006). The sensory conduction differs according to the voltages at which the cockroach is exposed. This ensures that
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