Calcium Pantothenate Assay - Microbiological Turbidimetric Vitamin B12 Assays

Calcium Pantothenate Assay 1
Microbiological turbidimetric vitamin B12 assays using Lactobacillus plantarum
Section 108 - BI209
Pharmaceutical Microbiology
Instructor: Andrei Bazyleu
Seyeon Jung
300988554
Centennial College
2018.08.06

Calcium Pantothenate Assay 2
Abstract
Calcium pantothenate assay is a microbial method used in finding the concentration of calcium
pantothenate in specific amino acids and vitamins. Calcium pantothenate is a salt from the family
of pantothenate acid that is ideal for human health since it is used in several functions of the
body including metabolism of carbohydrates and fats, cholesterol, steroid hormones alongside
other compounds.
In the laboratory, the quantity of pantothenate in B complex was estimated using vitamin B12
capsules with the quantities ranging between 2 and 10 ng/ml. The pantothenate solution was
already prepared in the laboratory priory carrying out the experiment. Lactobacillus plantarum
was used in the inoculation of the pantothenate-free growth media.
The procedure involved the addition of one drop of organism into all the tubes i.e. analyte, blank
and standard inoculated prior to their incubation at 35⁰C for 24 hours. The known concentrations
(a set of standards) were prepared and a set of unknowns were prepared and their transmittance
calculated by the end of the incubation period using a spectrophotometer with cuvettes. A
transmittance graph is used in the calculation of the concentration of the calcium pantothenate in
the media. The concentration of the calcium pantothenate in B in complex capsules is determined
using the curve.

Calcium Pantothenate Assay 3
Contents
Abstract............................................................................................................................................2
List of Figures..................................................................................................................................4
List of Tables...................................................................................................................................5
Introduction......................................................................................................................................6
Materials and Methods....................................................................................................................7
Pantothenate standard solution....................................................................................................7
Culture used.................................................................................................................................7
Preparation of the medium...........................................................................................................7
Samples used...............................................................................................................................7
Results..............................................................................................................................................8
Discussion......................................................................................................................................10
References......................................................................................................................................11

Calcium Pantothenate Assay 4
List of Figures
Figure 1: Standard curve showing the relationship between turbidimetric measurement (bacterial
growth) and calcium pantothenate concentration-8
Figure 2: Semi-log graph: Log of Concentration against Turbidimetric Measurement-9