Estimating Concentration of Calcium Pantothenate in Vitamin C Tablets

Running Head:
Name :Arshpreet kaur
Student no: 300960214
Course code:
Section :109
Date:
Partner’s name: Parimala vidya
TITLE
To Estimate the Concentration of Calcium Pantothenate in Commercial Sample of Vitamin C
Tablets by Using Turbidimetric Assay with Lactobacillus Plantrum.

1Pharmaceutical Lab Report
Abstract
Microbial approach which is employed to evaluate the concentration of the calcium
Pantothenate in particular amino acids and nutrients is known as Calcium Pantothenate test.
Calcium Pantothenate is a type of salt of the pantothenic corrosive that is vital for human
health. It is correlated with various capacities such as, digestion of sugars and fat, cholesterol,
combination of steroid hormones, and different absence and mixes of the nutrient, which can
causes extreme complication such as, kidney impairment, degeneration of spinal string and
mind, and so on.
In the laboratory, we evaluated the quantity of Pantothenate with the nutrient C cases
(quantities in the possibility of 10 ng/ml) in B complex. At that time the arrangement of
Pantothenate standard arrangement was organized in the laboratory. For the inoculation of the
sans Pantothenate development medium, Lactobacillus plantarum is used. Single drop of
Lactobacillus plantarum is supplemented in each of the standard containers, blank, analyte,
inoculated before hatching, which is done at 35° Celsius for about 24 hours. Several
processes (known fixations) were prepared and various questions were organized before
determining the transmittance, after the time frame of hatching by operating
spectrophotometer (wavelength 590nm) along with cuvettes. After that, the figure of
transmittance is evaluated, which helps in providing the combination of calcium Pantothenate
(due to bacterial infection, the media changes to turbid). To estimate the exact concentration
of (C18H32CaN2O10) calcium pantothenate in complex B cases, the curve is applied.

2Pharmaceutical Lab Report
Table of Contents
Introduction................................................................................................................................3
Materials and methods:..............................................................................................................4
Culture used...........................................................................................................................4
Pantothenate standard solution...............................................................................................4
Sample used...........................................................................................................................4
Sterile water...........................................................................................................................5
Sterile saline...........................................................................................................................5
Equipments:............................................................................................................................5
Method...................................................................................................................................5
Results:.......................................................................................................................................6
Figure 1..................................................................................................................................8
Figure 2..................................................................................................................................9
Discussion :................................................................................................................................9
References................................................................................................................................11

3Pharmaceutical Lab Report
Introduction
Pantothenic corrosive is also called B nutrient (nutrient B5) or Pantothenate. It is a
found by Roger J. Williams in the year 1919 and is soluble in water. It is used as basic
supplement for some living beings. Some living beings require the pantothenic corrosive to
incorporate CoA (coenzyme- A), and in addition it is also used for the assimilation of sugar,
protein and fats. It is the amide among the β-alanine and the β-alanine. Pantothenic acid is
found in nearly every nutrient, in some nutrient it is present in low quantity ad in some foods
such as, vegetables, meat, avocado, regal jam, yogurt, eggs and grain oats. It is commonly
found in the liquid sample, calcium Pantothenate and Provitamin Pantothenate. Pantothenic
corrosive is present as a fixing in healthy skin substances and hair. The isomer of the
pantothenic corrosive, D isomer (dextrorotatory) pose biologic movement. The effect of the
D isomer (dextrorotatory) is hindered by the (levorotatory) L. For this the free hydrated
medium is used, which is Pantothenate medium. In the medium all the essential nutrients are
present which are vital for the survival of Lactobacillus development. In addition,
development of Lactobacillus plantarum provides unusual reaction in present of the calcium
Pantothenate fixation and can be implemented by Turbidimetrically. Lactobacillus has
numerous different strains. One of the strain is Lactobacillus plantarum, which is generally
found in the ripened items and in different vital plant material.
It was first isolated from the saliva and is a gram positive bacteria. This gram positive
bacteria is beneficial for the human health such as, in skin.
Several procedures have been evaluated in performing the pantothenic corrosive test.
Measure of Calcium Pantothenate is applied in the research center in order to check the
combination of calcium Pantothenate. Calcium Pantothenate (C18H32CaN2O10 ) in the nutrient