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Abstract:
Antimicrobial agents are being used extremely in order to reduce massive losses
caused by E. coli infections (colibacillosis) in Pakistani poultry sector. In this study
100 avian morbific E. coli strains will be isolated from broiler chickens
with colisepticemia and will analyze for susceptibility to antimicrobials of veterinary
and human significance. In vitro antibiotic activities of 32 antibiotic substances
against strains will be determined by disc diffusion method (Kirby Bauer
method). Multiple resistances to antibiotics WILL BE observed in all the
isolates. The highest rate of resistance will be against Nalidixic
acid (100%), Lincomycin (100%). Resistance to Gentamicin will not observe and to
Amikacin, Cefazolin, Colistin, Tobramycin, Ceftizoxime, Cefixime, Lincospectin,
Ceftazidime and Florfenicol will low.
This study will show resistance against the antibiotic is directly related to the its
use. These results will confirm a substantial increase in the incidence of
antimicrobial resistance in the E. coli strains is most probably due
to increase in antibiotics as feed additives
for growth, promotion and prevention of diseases, treatment of diseases used
in poultry.
The present study recommends introduction of surveillance programs to monitor
antimicrobial resistance in pathogenic bacteria is strongly needed because other
than animal health problems, transmission of resistant clones and resistance plasmids
of E. coli from animals i.e. poultry to humans can occur.
Keywords: Antibiotics susceptibility, Escherichia coli, chicken.
1. Introduction:
Escherichia coli is the most important agent causing secondary
bacterial infection in poultry and may also be a primary pathogen (Gross, 1994).
Colibacillosis is the most frequently reported disease in surveys of poultry diseases or
condemnations at processing (Saif, 2003). Colibacillosis refers to any localized or
systemic infection caused entirely or partly by avian pathogenic Escherichia coli
(APEC), including colisepticemia, coligranuloma (Hjarre’s disease), air sac disease
(chronic respiratory disease, CRD), cellulites (inflammatory process), swollen-head
syndrome, peritonitis, salpingitis, osteomyelitis/synovitis (turkey osteomyelitis
complex), panophthalmitis, and omphalitis/yolk sac infection (Saif, 2003).
Colisepticemia is the Well-known form colibacillosis and cause hugeeconomic losses
in aviculture throughout the world (Barnes and Gross, 1997; Ewers, et al., 2003). In
the past few years, both the incidence and severity of colibacillosis have increased
rapidly, and current trends indicate that it is probably to continue and become an even
greater problem in the poultry industry (Altekruse et al., 2002; Blanco, 1997).

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