KJC12 Antibody and its Impact on p53 Isoforms Gene Expression

Running Header; Lab Report
UNIVERSITY:
NAME :
STUDENT ID:
COURSE CODE
COURSE NAME
ASSIGNMENT

WORKSHOP REPORT (BS32029)
Abstract
P53 isoforms are the tumour suppressors and inducers which affect cancer prognosis in
the body. It has different pathways in the body and can either induce cell cycle arrests or
proliferate cancer cells. The activities of p53 isoforms are due to the properties of the DNA
and mRNA transcription. In cancer cells, p53 have been found to be mutant genes in cancer
tissues. P53 encodes different forms of protein isoforms, which bind and not easily expressed.
The family of p53 has been found to have 12 different forms displaying differential tissue
expression properties. This experiment shows the ability of TP 53 gene to encode the p53
proteins. This is affected by the presence of promoters which generates Δ133p53, leading to
full length expression. These forms have been found to have an expression in mRNA levels at
different degrees on different types of tissues both cancerous and normal tissues. The p53
isoforms have different forms of specificity which can be crucial in selective regulation in
establishing clinical outcomes. This experiment has established the effect of KJC12 antibody
on p53 isoforms gene expression. The objective is to determine the impact of KJC12
specificity on different p53 isoforms linked to cancer. Antibodies have been utilized to
exonerate the different specifies of the p53 isoforms. The analysis of KJC12 antibody has
shown to be key in expressing the different forma of the p53 isoforms which are crucial in
cancer marker cells, prognosis, management and clinical outcomes. Compared to other
antibodies, KJC12 was able to express most of the p53 isoforms thus showing the relevance it
has in identifying cancer specific is forms which is a crucial breakthrough in cancer
medication and therapy management.
2

WORKSHOP REPORT (BS32029)
Introduction
The relevance of biomarkers which include cell cycle regulators, pro-apoptotic ,
markers of angio genesis and proliferation marker cells have been found to be significant in
cancer tumour cells.1 P53 has been part of these markers and plays a crucial role in
maintaining the integrity of the cells. P53 is produced through tumour suppressor gene. The
activation of p53 is initiated when the cells surrounding it are damaged or stressed. When
activated the p53 proteins initiate cell cycle arrest and DNA repair. In other cases it cases cell
death. Thus the critical aspect in understanding p53 is the gene expression in tumour
formation and its role in cancer formation when subjected to different antibodies. Thus there
is need to find out how p53 isoforms can be expressed and how this expression is affected by
antibodies which are be linked to outcomes in clinical arena and its role in cancer patient
treatment. 2 The objective is to determine the impact of KJC12 specificity on different p53
isoforms linked to cancer.
The functioning of the p53 isoforms pathway in cells is not well understood in cancers
though avenues such as mutation process or interaction with proteins. Current knowledge has
grabbled with question of linking p53 mutations state and cancer treatment protocols and
expected clinical outcomes among cancer patients, this signify that the functioning of p53
cycle has not be well established. 3 P53 has been shown to express itself in not only 1 form
but in 12 different isoforms which is linked to splicing, translation initiation and promotive
usage.
Thus it is acknowledged that p53 isoform expression is associated with cancer
prognosis, leading to the relationship which p53 mutation treatment process and cancer
outcomes to be largely un understood. Its molecular mechanism is well established. In this
experimental study, we analysed how the biological activities of p53 isoforms are impaired
by specific antibodies.4 This seeks to establish how p53 isoforms is expressed on p53
pathway and how this impacts on cancer treatment and overall ageing process.
This experiment thus analyses the biological effects of the different inhibition of p53
isoforms, which can interact with specific antibody referred to as KJC12 at protein level
expression in human tissues both in cancer and normal tissues.
Results
The first experiment analysis entailed transfections of H1299 lung cancer cells being
subjected to p53 isoform expression. Transfections of p53 proteins (DNA), in H1299 cells
which are human non small cell lung carcinoma cells derived from lymph node. H1299 cells
have the ability to divide indefinitely. They have homozygous properties on TP53 cells which
3