Measurement of Amino Acids in Plasma using HPLC

Laboratory Report

Table of Contents
INTRODUCTION...........................................................................................................................1
BODY..............................................................................................................................................2
What are the different aspects of measurement of amino acids in plasma using HPLC?...........2
Compare key HPLC methodologies............................................................................................4
CONCLUSION..............................................................................................................................11
REFERENCES..............................................................................................................................12

INTRODUCTION
High performance liquid chromatography refers to an improved form of column
chromatography which is known as an effective tool of conducting analysis of particular solvent.
It includes the criterion of permitting the utilisation of much small size of particles in context of
column packing material that provides sufficiently greater surface region regarding interactions
between molecules flowing and stationary phase (Mant and Hodges, 2017). However, there are
various kinds of chromatographic approaches exist in which suitable one can be applied in
medical field to gain accurate outcomes. Moreover, the levels of amino acids can be evaluated
with the help of chromatographic method which facilitate to determine increased or decreased
levels in order to identify presence of specific health problem.
The measurements of amino acids in plasma is important because increased level of
specific amino acid is known to be strong symbol in regards to presence of particular problems.
This issue is related to the capability of human body in order to break down that specific amino
acid. It is also used to determine the reduced levels of amino acids in blood which usually occurs
in the situation of inadequate nutrition, fever and other several medical circumstances
accordingly. Meanwhile, the amino acids are required to be present in appropriate manner which
is necessary to ensure normal health of an individual.
There are number of different chromatographic approaches which can be utilised for
measuring amino acids that are mentioned here. It includes liquid chromatography, gas
chromatography, thin layer chromatography, paper chromatography, column chromatography,
high performance liquid chromatography, fast protein liquid chromatography, superficial fluid
chromatography, affinity chromatography, reversed phase chromatography, two dimensional,
pyrolysis gas and counter current chromatography (Zeeb, Farahani and Papan, 2016). The
different detection methods are UV-Vis spectroscopy, Infrared spectroscopy, Pre-column
Derivatization Method, Post-column Derivatization Method etc.
The problem in conducting measurement of amino acids in plasma with the help of
chromatography consist improper conduct of whole procedure. It includes the format that patient
should not eat at at least 4 hours before taking blood sample to measure amino acids otherwise
the results may be inaccurate.
The present review will focus on measurement of amino acids in plasma with the help of
high performance liquid chromatography including entire procedure. It will include the way of
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