Protein Discovery and Analysis PDF

Running head: PROTEIN DISCOVERY AND ANALYSIS
BETA GLUCORINIDASE
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PROTEIN DISCOVERY AND ANALYSIS1
Table of Contents
Introduction................................................................................................................................2
The primary structure of PET protein........................................................................................2
The physico-chemical properties of PET protein.......................................................................3
Initial purification of the protein................................................................................................8
Present purification of the protein..............................................................................................9
Bibliography.............................................................................................................................11

PROTEIN DISCOVERY AND ANALYSIS2
Introduction
Human beta glucuronidase has been defied as a type of glucuronidase which catalyzes the
hydrolysis of beta D glucuronic acid residue that has been found derived from a non-reducing
mutopolysaccharide in nature. This protein has been found to be located in the lysozyme.
Beta glucuronidase has been found to be secreted from gusB gene of human (Di et al., 2017).
The protein is synthesised in the form of a monomer of 80 kDa size before proteolysis. After
proteolysis, it has been found to be broken down to 78 kDa monomer, which is the active
protein.
The primary structure of PET protein
10 20 30 40 50
MARGSAVAWA ALGPLLWGCA LGLQGGMLYP QESPSRECKE LDGLWSFRAD
60 70 80 90 100
FSDNRRRGFE EQWYRRPLWE SGPTVDMPVP SSFNDISQDW RLRHFVGWVW
110 120 130 140 150
YEREVILPER WTQDLRTRVV LRIGSAHSYA IVWVNGVDTL EHEGGYLPFE
160 170 180 190 200
ADISNLVQVG PLPSRLRITI AINNTLTPTT LPPGTIQYLT DTSKYPKGYF
210 220 230 240 250
VQNTYFDFFN YAGLQRSVLL YTTPTTYIDD ITVTTSVEQD SGLVNYQISV
260 270 280 290 300
KGSNLFKLEV RLLDAENKVV ANGTGTQGQL KVPGVSLWWP YLMHERPAYL
310 320 330 340 350
YSLEVQLTAQ TSLGPVSDFY TLPVGIRTVA VTKSQFLING KPFYFHGVNK
360 370 380 390 400
HEDADIRGKG FDWPLLVKDF NLLRWLGANA FRTSHYPYAE EVMQMCDRYG
410 420 430 440 450
IVVIDECPGV GLALPQFFNN VSLHHHMQVM EEVVRRDKNH PAVVMWSVAN
460 470 480 490 500
EPASHLESAG YYLKMVIAHT KSLDPSRPVT FVSNSNYAAD KGAPYVDVIC
510 520 530 540 550
LNSYYSWYHD YGHLELIQLQ LATQFENWYK KYQKPIIQSE YGAETIAGFH
560 570 580 590 600
QDPPLMFTEE YQKSLLEQYH LGLDQKRRKY VVGELIWNFA DFMTEQSPTR
610 620 630 640 650
VLGNKKGIFT RQRQPKSAAF LLRERYWKIA NETRYPHSVA KSQCLENSLF
T

PROTEIN DISCOVERY AND ANALYSIS3