LACTOFERRIN Medical Question and Answer 2022

Running head: MEDICAL
LACTOFERRIN
Name of the Student
Name of the University
Author Note

MEDICAL1
Table of Contents
Answer 1....................................................................................................................................2
Answer 2....................................................................................................................................2
ProtScale..................................................................................................................................4
User-provided sequence:...................................................................................................4
Answer 3....................................................................................................................................6
Answer 4....................................................................................................................................7
Bibliography...............................................................................................................................9

MEDICAL2
Introduction
Lactoferrin has been defined as a multifunctional, globular glycoprotein that has been widely
found inside secretory fluids including tears, milk and saliva. Human colostrum has been
found to contain the highest concentration of lactoferrin. This can be purified from milk by
the use of various chromatographic techniques. This paper will throw a light on the amino
acid composition of lactoferrin including its primary structure and the past and present
purification steps.
Answer 1
Primary structure of Lactoferrin with domains and subdomains, and disulfide
connections with glycosylated amino acids if present:
N-terminal end-
MKLVFLVLLFLGALGLCLAGRRRRSVQWCAVSQPEATKCFQWQRNMRRVRGPPVS
CIKRDSPIQCIQAIAENRADAVTLDGGFIYEAGLAPYKLRPVAAEVYGTERQPRTHYY
AVAVVKKGGSFQLNELQGLKSCHTGLRRNAGWNVPIGTLRPFLNWTGPPEPIAVAR
FFSASCVPGADKGQFPNLCRLCAGTGENKCAFSSQEPYFSYSGAFKCLRDGADVAFI
RESTVFEDLSDEAERDEYELLCPDNTRKPVDKFKDCHLARVPSHAVVARSVNGKED
AIWNLLRQAQEKFGKDKSPKFQLFGSPSGQKDLLFKDSAIGFSRVPPRIDSGLYLGSG
YFTAIQNLRKSEEEVAARRARVVWCAVGEQELRKCNQWSGLSEGSVTCSSASTTED
CIALVLKGEADAMSLDGGYVYTAGKCGLVPVLAENYKSQQSSDPDPNCVDRVEGY
LAVAVVRRSDTSLTWNSVKGKKSCHTAVDRTAGWNIPMGLLFNQTGSCKFDEYFS
QSCAPGSDPRSNLCALCIGDEQGENKCVPNSNERYYGYTGAFRCLAEDAGDAFVKG
VTVLQNTDGNNNEAWAKDLKLADFALLCLDGKRKPVTEARSCHLAMAPNHAVVS
RMDKVERLKQVLLHQQAKFGRNGSDCPDKFCLFQSETKNLLFNDNTECLARLHGKT
TYEKYLGPQYVAGITNLKKCSTS PLLEACEFL K- C terminal end
Yellow domain= Transferrin like domain 1H
Blue domain= Transferring like domain 2
No glycosylation or disulphide bridges have been observed in the primary structure of
lactoferrin.

MEDICAL3
Answer 2
Mature protein sequence
20 30 40 50
G RRRRSVQWCA VSQPEATKCF QWQRNMRRVR
60 70 80 90 100
GPPVSCIKRD SPIQCIQAIA ENRADAVTLD GGFIYEAGLA PYKLRPVAAE
110 120 130 140 150
VYGTERQPRT HYYAVAVVKK GGSFQLNELQ GLKSCHTGLR RNAGWNVPIG
160 170 180 190 200
TLRPFLNWTG PPEPIEAAVA RFFSASCVPG ADKGQFPNLC RLCAGTGENK
210 220 230 240 250
CAFSSQEPYF SYSGAFKCLR DGAGDVAFIR ESTVFEDLSD EAERDEYELL
260 270 280 290 300
CPDNTRKPVD KFKDCHLARV PSHAVVARSV NGKEDAIWNL LRQAQEKFGK
310 320 330 340 350
DKSPKFQLFG SPSGQKDLLF KDSAIGFSRV PPRIDSGLYL GSGYFTAIQN
360 370 380 390 400
LRKSEEEVAA RRARVVWCAV GEQELRKCNQ WSGLSEGSVT CSSASTTEDC
410 420 430 440 450
IALVLKGEAD AMSLDGGYVY TAGKCGLVPV LAENYKSQQS SDPDPNCVDR
460 470 480 490 500
PVEGYLAVAV VRRSDTSLTW NSVKGKKSCH TAVDRTAGWN IPMGLLFNQT
510 520 530 540 550
GSCKFDEYFS QSCAPGSDPR SNLCALCIGD EQGENKCVPN SNERYYGYTG
560 570 580 590 600
AFRCLAEDAG DVAFVKGVTV LQNTDGNNNE AWAKDLKLAD FALLCLDGKR
610 620 630 640 650
KPVTEARSCH LAMAPNHAVV SRMDKVERLK QVLLHQQAKF GRNGSDCPDK
660 670 680 690 700
FCLFQSETKN LLFNDNTECL ARLHGKTTYE KYLGPQYVAG ITNLKKCSTS
710
PLLEACEFLR K