Reverse Phase Liquid Chromatography

REVERSE PHASE CHROMATOGRAPHY1
REVERSE PHASE LIQUID CHROMATOGRAPHY
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REVERSE PHASE CHROMATOGRAPHY2
Table of Content
Introduction...................................................................................................3
Discussion .................................................................................................4
Conclusion.....................................................................................................8
Bibliography..................................................................................................9

REVERSE PHASE CHROMATOGRAPHY3
Introduction
Reverse phase liquid chromatography is Reverse phase chromatomatagraphic technique
that separate nonpolar molecules in a solution. Nonpolar solvents such as nitrile are used as the
stationary phase used is nonpolar while the polar solvents are the moving phase (Gocan,
Cimpan, and Comer, 2016,p.90). While in the normal phase the theory of separation of particles
is based on the interaction between the polar matrix (silica beads) and the nonpolar mobile phase
(nonpolar solvent).It is viewed to be superior to the other modes of chromatographic techniques,
such as the thin layer chromatography, paper chromatography, gas chromatography among many
different methods as it can analyze a variety of target compounds.