Spectroscopy and Analytical Chemistry (CHM2922) - Lab Reports

Spectroscopy and Analytical
Chemistry (CHM2922) - Lab Reports
Chemistry
Monash University, Malaysia Campus
65 pag.
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Spectroscopy and
Analytical Chemistry
(CHM2922) – Lab
Reports
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SCHOOL OF SCIENCE
ASSESSMENT COVER
SHEET
Student’s name (Surname)
Amran (Given names)
Nuramira Batrisyia
ID number 27467236 Phone
Unit name Spectroscopy and Analytical
Chemistry
Unit code CHM2922
t
Note: If this is a group assignment, please include the names of all other group members.
Title of
assignment
Experiment 1: Gas Chromatography - Measuring the Alcohol Content
of Wine
Lecturer/tutor Assoc Prof Dr Lim Yau Yan
Is this an authorisedgroup assignment? Yes No
Has any part of this assignment been previously submitted as part
ofanotherunit/course? Yes No
Tutorial/laboratory day &
time
Tuesday, 2PM
Due dateTuesday, 2PM Date submittedT u e s d a y
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with the signature of the lecturer/tutor.
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lecturer/tutor.................................................
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 I certify that I have not plagiarised the work of others or participated in unauthorised
collaboration when preparingthisassignment. 8/8/2020
Signature..................................................... Date……………………………………
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RISK
ASSESSMENT
Name: Nuramira Amran
Lab Course details (e.g.
CHM
2922)
CHM2922 Date 5/8/2020
Experiment name and
number
Experiment 1: Gas Chromatography – Measuring the
Alcohol
Content ofWine
Identify the
HAZARD
(the POTENTIAL to do
harm)
Determine the RISK
(the PROBABILITY that
harm mayresult)
CONTROL the Risk
(PREVENTING an
incident)
Absolute ethanol Highly flammable. Causes
eye
irritation.
Handle in fumehood. Wear
protective gloves and
goggles
Acetone
Highly flammable. Causes
eye irritation. May cause
drowsiness
or dizziness.
Handle in fumehood.
Wear protective gloves
and goggles.
Glassware Cuts, stab wound from
sharp edges
Handle with care, dispose
of
broken glass using a
dustpan & brush. If cut,
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CHM2922Laboratory Report Name:NuramiraAmran
EXPERIMENT1:
GASCHROMATOGRAPHY– MEASURING THEALCOHOLCONTENT OFWINE
AIM:
 Todeterminethecontentofwinebygaschromatographyusingtwomethodsofquantif
cation: by the calibration and internal standardmethods.
 To determine the chromatographic elution parameters: capacity factor
andresolution.
DETAILS OF INSTRUMENT USED AND WHY IT IS SELECTED FOR THIS EXPERIMENT:
According to Staufer et al. (2008), gas-liquid chromatography is defned as “a
specifc type of chromatography that utilizes an inert gaseous mobile phase
and a liquid stationary phase”. The instrument would separate mixtures and
determine the amount of each component. It is selected for this experiment
because it could separate volatile samples such as acetone and ethanol which
could easily vaporise.
EXPERIMENTALSECTION: (Briefly summarise the procedures < 150 words)
The calibration starts with the preparation of standard solutions of ethanol in
water with concentrations of 5, 10, 15 and 20 % (v/v) ethanol. The peak areas
for ethanol peaks were measured and recorded. The internal standard method
starts with the preparation of an acetone standard,which contains 15 % (v/v) of ethanol
and acetone. For each wine sample aspecifc amount of the acetone standardwasaddedtomake15%(v/
v)anddilutedtothemarkwiththesample.Thesesampleswould be called acetone-spiked
samples. Next, 1 µL injections of 15 % (v/v) ethanol - 15 % (v/v) acetone
standardandoftheacetone-
spikedwinesamplesweremade.Todeterminetheaccuracy,theQCwas provided, with
15 % (v/v) ethanol and 15 % (v/v) acetone. The results obtained from the QC was
compared with results obtained from the 15 % (v/v) ethanol – 15 % (v/v)
acetonestandard.
/
SUMMARY OFRESULTS:
Table 1: Peak elution data
tM= 0.16 mins
tR(ethanol) = 1.889
mins
t’R(ethanol) = 1.729
mins
k’ (ethanol) =
10.8064.1
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/
tR(acetone) = 1.398
mins
t’R(acetone) = 1.238
mins
k’ (acetone) =
7.7375
w (ethanol) = 0.1
mins
w (acetone) = 0.1
mins
R = 4.91
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2of
8
/
/
/
Ethanol Concentration % (v/v)
2520051015
y = 743.41x - 142.45
R² = 0.9996
16000
14000
12000
10000
8000
6000
4000
2000
0
Calibration Graph of Peak Area vs Diferent
Concentration of Ethanol Standard Solution
Table 2: Calibration data
Ethanol
concentrat
ion
%(v/v)
Peak
area 1
Peak
area 2
Peak
area 3
Mean
area
Standa
rd
deviati
on
% RSD
5 3374.06 3586.62 14504.39 3541 15
0
4.
2
10 7339.71 7408.66 11224.34 7277 17
3
2.
4
15 11426.4 10766.45 7081.15 11139 33
8
3.
0
20 14492.62 14935.07 3663.25 14644 25
2
1.
7
Calibration Graph
Figure1:Calibrationgraphofpeakareasvsethanolindiferentconcentration,5%,10
%,15% and 20%
Peak Area6.1
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