Biochemical Fractionation of Milk Proteins

SLE222 Biochemical Metabolism T2 2019 Worksheet B
Worksheet B (covers practical 2)
Biochemical fractionation of milk proteins
Name:
Prac
Partners:
Prac date:
Due date:
1) Electronic Format
The worksheet must be produced entirely in an electronic fashion, however, we do not
accept ePens. Hand-written or hand-drawn components, be it as original or as scanned
image, will not be accepted/not receive any marks! Submit electronically as either a Word
or PDF file via the designated CloudDeakin dropbox.
2) Plagiarism and Collusion
Before submitting your worksheet to the dropbox submit it to the Check Your Work: Turnitin
Assignment Folder available on CloudDeakin. In case that we find that work is the result of
plagiarism (from foreign source like internet or fellow student) or collusion we report this to
the Faculty Academic Progress and Discipline Committee.Note: It is NOT sufficient to submit your worksheet only to the Turnitin Folder! Your
worksheet will be marked only if you submit it to the appropriate Dropbox!
3) Due dates for worksheet submission:
Worksheet A is due 5.00 pm two teaching weeks after practical session 1, this is the day
BEFORE your prac 2
Worksheet B is due 5.00 pm two teaching weeks after practical session 2, this is the day
BEFORE your prac 3
Worksheet C is due 5.00 pm two teaching weeks after practical session 4 (!), this is the day
BEFORE your prac 5
Worksheet D is due 5.00 pm ONE (!) teaching week after practical session 5 (!)
4) Referencing
All foreign sources of information, i.e. information that was not
provided in this unit, must be cited. The citation style to be used is Harvard
style, for more information about this style and referencing in general see:
https://www.deakin.edu.au/students/studying/study-support/referencing. Citations will be
considered only when they are placed as in-text citations such that it is clear which
statement they do support. Furthermore, bibliographic details of a citation must be listed
within the question where they are used, reference lists at the end of the worksheet will not
be accepted. The citation needs to be a quality journal article, book or refereed
website. No marks are awarded if Wikipedia or poor-quality websites (eg. the
website of a course at another institution) are cited.
The practical manual, the prac script, OLMs and lecture notes can NOT be
cited. Citations of text books are often most appropriate for the level of
this unit and therefore encouraged. If you do cite a textbook you MUST
indicate relevant page numbers.
YOU MUST NOT DELETE ANY TEXT FROM THE
WORKSHEET TEMPLATE,
HOWEVER IT IS ACCEPTABLE TO ADJUST THE SIZE OF
THE ANSWER BOXES

SLE222 Biochemical Metabolism T2 2019 Worksheet B
5) Learning Outcomes:
Q1, Q2a:
Written communication. Presentation of experimental data.
Q3:
Quantification skills. Application of a mathematical equation to solve a
typical problem encountered in the biochemistry lab.
Q2b,c, Q4:Critical thinking & problem solving. Use of logic and discipline
specific knowledge to describe and predict an experimental outcome
YOU MUST NOT DELETE ANY TEXT FROM THE
WORKSHEET TEMPLATE,
HOWEVER IT IS ACCEPTABLE TO ADJUST THE SIZE OF
THE ANSWER BOXES

SLE222 Biochemical Metabolism T2 2019 Worksheet B
1a. Produce a figure using the image of the SDS-PAGE gel that you
created in the prac
(1.5 marks)
- Only the lane for the molecular mass standards and the skim milk
sample lane are to be presented. You will be given 0 marks for Q1a if
other or additional lanes are presented! You can cut and paste the
two required lanes out of the original gel image file.
Explanatory note: The teaching team strictly enforces the above indicated
requirements for presentation of SDS PAGE data to ensure the originality of 2019
worksheet submissions.
- Indicate the identity of the lanes (what has been loaded in which lane), the
origin of electrophoresis (ie the bottom of the well into which the protein was
loaded), electrophoresis front (the most advanced part of the electrophoresis
in the gel), the molecular mass of markers proteins and indicate the casein
bands and heat resistant low molecular mass proteins. You can use e.g.
Power-Point, Photoshop or Illustrator or similar software for his task.
- Write a concise figure legend with a minimal number of words that allows the
reader to understand what they see. Note: A figure legend gives some
experimental information to provide context but does not reproduce the entire
procedure in detail. It also defines any terms or symbols or describes equation
terms to allow the reader to understand the figure by itself. A figure legend
should not contain any interpretation of the results!
YOU MUST NOT DELETE ANY TEXT FROM THE
WORKSHEET TEMPLATE,
HOWEVER IT IS ACCEPTABLE TO ADJUST THE SIZE OF
THE ANSWER BOXES