Assignment About Biochemistry

SLE212 Biochemistry T1 2020 Assessment Worksheet A
Worksheet A (covers practical 1)
Name
Prac
Partners
Prac date
Due date
1) Electronic Format
The worksheet must be produced entirely in an electronic fashion, however, we do not
accept ePens! Hand-written or hand-drawn components, be it as original or as scanned
image, will not be accepted/not receive any marks! Submit electronically as either a Word
or PDF file via the designated Deakin Sync dropbox.
2) Plagiarism and Collusion
Before submitting your worksheet to the dropbox submit it to the Check Your Work: Turnitin
Assignment Folder available on CloudDeakin. In case that we find that work is the result of
plagiarism (from a foreign source like the internet, or from a fellow student) or collusion we
will report this to the Faculty Academic Progress and Discipline Committee.Note: It is NOT sufficient to submit your worksheet only to the Turnitin Folder! Your
worksheet will be marked only if you submit it to the appropriate Dropbox!
3) Due dates for worksheet submission:
Worksheet A is due 5:00 pm two teaching weeks after practical session 1, this is the day
BEFORE your prac 2
Worksheet B is due 5:00 pm two teaching weeks after practical session 2, this is the day
BEFORE your prac 3
Worksheet C is due 5:00 pm two teaching weeks after practical session 4 (!), this is the day
BEFORE your prac 5
Worksheet D is due 5:00 pm ONE (!) teaching week after practical session 5 (!)
4) Referencing
All foreign sources of information, i.e. information that was not
provided in this unit, must be cited. The citation style to be used is Harvard
style, for more information about this style and referencing in general see:
https://www.deakin.edu.au/students/studying/study-support/referencing. Citations will be
considered only when they are placed as in-text citations such that it is clear which
statement they do support. Furthermore, bibliographic details of a citation must be listed
within the question where they are used, reference lists at the end of the worksheet will not
be accepted! The citation needs to be a quality journal article, book or refereed
website. No marks are awarded if Wikipedia or poor-quality websites (eg. the
website of a course at another institution) are cited.
The practical manual, the prac script, OLMs and lecture notes CANNOT be
cited! Citations of text books are often most appropriate for the level of
this unit and therefore encouraged! If you do cite a textbook you MUST
indicate relevant page numbers!
5) Learning Outcomes:
Q1: Written communication. Presentation of experimental data.
Q2, Q6: Quantification skills. Application of a mathematical equation to
quantitatively describe a system.
DO NOT DELETE ANY TEXT FROM THE
QUESTIONS

SLE212 Biochemistry T1 2020 Assessment Worksheet A
Q3: Discipline specific knowldege
Q4, Q5:Critical thinking & problem solving. Use of logic and discipline specific
knowledge to describe and predict an experimental outcome
DO NOT DELETE ANY TEXT FROM THE
QUESTIONS

SLE212 Biochemistry T1 2020 Assessment Worksheet A
1. Generate a Standard Curve.
Use
Excel to graph a marked scatter plot (this means only data points are shown
in the graph and the points are not connected by a line) of the standard values
that you obtained for glycine. Draw a linear regression line using absorbance
values vs concentration (in mM) for each standard. Plot the independent variable
on the x-axis and the dependent variable on the y-axis. Display the straight-line
equation of the regression line on the graph. Write this equation in terms of the
definition of the variables (eg. Replace “x” with “Glycine concentration” etc).
IMPORTANT: The colour of the regression line and the data points MUST
be ORANGE (you will receive 0 marks for question 1, if a different colour
is shown).
Explanatory note: The teaching team strictly enforce the above indicated color
requirements in order to ensure the originality of 2020 worksheet submissions.
Give the graph a concise, useful and descriptive title, and label the axes. Include
appropriate units or cite the wavelength measured. Provide a figure legend,
which concisely describes the graph that is shown, and the experiment that
underlies the measurements. A figure legend does not reproduce the entire
experimental procedures in detail, but rather puts the depicted result into an
experimental context. In the figure legend define the terms of the equation that
is presented in the graph. 2 marks
DO NOT DELETE ANY TEXT FROM THE
QUESTIONS

SLE212 Biochemistry T1 2020 Assessment Worksheet A
2. Using your standard curve and straight line equation generated in
question 1, calculate the absorbance of a sample which has a
concentration of 57 μM.
i) Write down the equation derived from your Excel generated standard curve
(question 1) and describe its components including all units (!); ii) show all
details of the working out of your calculation. Indicate all units! Provide the
answer with three (!) decimal precision, iii) state the final answer in a full
sentence. (note: avoid mathematical symbols [=, +, -, etc] in your answer
sentence).
1 mark
DO NOT DELETE ANY TEXT FROM THE
QUESTIONS
Insert/ Cut and paste your standard curve in this space. Do NOT insert a scan
from a hand-drawn graph! The graph must be generated with suitable
graphing software!
MUST be excel/software generated and has to include software generated
regression line
The equation of this graph is y=4.8983x + c. The y is the absorbance and x is
the Glycine concentration(mM). C is the y-intercept where it croses the y axis,
which is equal to 0 because the trendline pass through the 0 y-intercept. This
graph shows the relationship between concentration and absorbance. When
the concentration increases, the absorbance increases.The graph shows that
is positively correlated because the graph is sloping upwards.