Protein Discovery And Analysis

Running head: PROTEIN DISCOVERY AND ANALYSIS
GROWTH HORMONE
Name of the Student
Name of the University
Author Note

2
Table of Contents
Introduction................................................................................................................................3
The primary structure:................................................................................................................3
Physico-chemical properties of the protein:...............................................................................3
Matured peptide sequence......................................................................................................3
Amino acid composition with pI and MW.............................................................................4
Hydropathy plot.....................................................................................................................5
Historical purification step.........................................................................................................7
Present purification process.......................................................................................................8
Bibliography...............................................................................................................................9

3
Introduction
Growth hormone has been stated as the protein hormone which is secreted as 190 amino acid
containing protein. This hormone is synthesized from pituitary cells known as Somatotrophs.
This hormone us a major participant of various complex process of human physiology
including both metabolism and growth. This paper will discuss the primary structure,
physico-chemical properties and purification processes of growth hormone (somatotropin).
The primary structure:
10 20 30 40 50
MATGSRTSLL LAFGLLCLPW LQEGSAFPTI PLSRLFDNAM LRAHRLHQLA
60 70 80 90 100
FDTYQEFEEA YIPKEQKYSF LQNPQTSLCF SESIPTPSNR EETQQKSNLE
110 120 130 140 150
LLRISLLLIQ SWLEPVQFLR SVFANSLVYG ASDSNVYDLL KDLEEGIQTL
160 170 180 190 200
MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD ALLKNYGLLY CFRKDMDKVE
210
TFLRIVQCRS VEGSCGF
Features Positions with respective color
Signal peptide 1-26, Red
Mature peptide chain 27-217, Green
Disulfide bond 79-191, Pink
Disulfide bond 208-215, Pink
Physico-chemical properties of the protein:
Matured peptide sequence
>sp|P01241|SOMA_HUMAN Somatotropin OS=Homo sapiens OX=9606 GN=GH1 PE=1 SV=2