This essay discusses the importance of workplace culture in practice development in healthcare organizations. It covers the theoretical implementation of workplace culture, practical implications, and its role in practice development principles. The essay emphasizes the significance of person-centered care, staff satisfaction and retention, and patient outcome in the effective workplace culture. It also highlights the impact of leadership style, communication, innovation, and stakeholder involvement in building an effective workplace culture. The essay concludes that the effective workplace culture is imperative for healthcare firms to enhance their service quality by elevating the employees’ satisfaction and positively influencing the patient outcome.