Biotechnology and Genetic Engineering Reviews

Running head: MEDICAL
HIRUDIN
Name of the Student
Name of the University
Author Note

MEDICAL1
Table of Contents
Answer 1....................................................................................................................................2
Answer 2....................................................................................................................................3
Answer 3....................................................................................................................................6
Answer 4....................................................................................................................................7
Bibliography...............................................................................................................................9

MEDICAL2
Answer 1
The primary structure of Hirudin
N’ terminal end- V-V-Y-T-D-C-T-E-S-G-Q-N-L-C-L-C-E-G-S-N-V-C-G-Q-G-N-K-C-I-L-
G-S-D-G-E-K-N-Q-C-V-T-G-E-G-T-P-K-P-Q-S-H-N-D-G-D-F-E-E-P-E-E-Y-L-Q- C’
terminal end
Important domains and sub-regions
N’ terminal end- V-V-Y-T-D-C-T-E-S-G-Q-N-L-C-L-C-E-G-S-N-V-C-G-Q-G-N-K-C-I-L-
G-S-D-G-E-K-N-Q-C-V-T-G-E-G-T-P-K-P-Q-S-H-N-D-G-D-F-E-E-P-E-E-Y-L-Q- C’
terminal end
In the above structure, there are two domains marked with yellow and red starting from the N
terminal end and the C terminal end respectively.
Domain 1 (Yellow)= Position 1 to 3
Domain 1 is responsible for the interaction of the protein with the active sites of thrombin.
Domain 2 (Red)= Position 55 to 65
Domain 2 is responsible for the interaction with fibrinogen binding exosite of thrombin.
Indication of disulphide connections and glycosylated amino acids
N’ terminal end- V-V-Y-T-D-C-T-E-S-G-Q-N-L-C-L-C-E-G-S-N-V-C-G-Q-G-N-K-C-I-L-
G-S-D-G-E-K-N-Q-C-V-T-G-E-G-T-P-K-P-Q-S-H-N-D-G-D-F-E-E-P-E-E-Y-L-Q- C’
terminal end.
-S-S-
-S-S-
-S-S-

MEDICAL3
Disulphide bond linkages-
Positions located in the primary sequence:
1. Position 6 to 14= Red
2. Position 16 to 28= Green
3. Position 22 to 39= Grey
Glycosylation is visible at 45th Threonine residue (O-linked glycosylation).
Answer 2
ExPASy analysis
The mature protein sequence is –
N’ terminal end- V-V-Y-T-D-C-T-E-S-G-Q-N-L-C-L-C-E-G-S-N-V-C-G-Q-G-N-K-C-I-L-
G-S-D-G-E-K-N-Q-C-V-T-G-E-G-T-P-K-P-Q-S-H-N-D-G-D-F-E-E-P-E-E-Y-L-Q- C’
terminal end
Glycoprotein
O linked glycosylation visible at the Threonine residue and thus the protein has O linked or
Oxygen linked glycosylation.
Physico-chemical property of the protein
Hirudin is an acidic protein since it contains more number of negatively charged amino acids
than the positively charged ones. The most significant chemical property of Hirudin is that it
is a 64 amino acid polypeptide which weighs 7000 kDa on an average.
Number of amino acids: 65
Molecular weight: 6969.53
Theoretical pI: 4.04