Biology Laboratory Report 2022

Running head: BIOLOGY
LABORATORY REPORT
Name of the Student
Name of the University
Author Note

BIOLOGY1
Table of Contents
Introduction................................................................................................................................3
Materials and Methods...............................................................................................................3
Materials required..................................................................................................................3
Method used...........................................................................................................................4
Procedure................................................................................................................................5
Data analysis..........................................................................................................................6
Results........................................................................................................................................6
DNS test.................................................................................................................................6
Table of absorbance values................................................................................................6
Graphical analysis..............................................................................................................7
GOD test.................................................................................................................................8
Table of absorbance values................................................................................................8
Graphical analysis..............................................................................................................9
Discussion................................................................................................................................10
References................................................................................................................................11

BIOLOGY2
Introduction
Glucose has been defined as a simple sugar that has a molecular formula of
C6H12O6. The compound is an abundant monosaccharide associated with a subcategory of
carbohydrates that is mainly made by most algae and plants during their process of
photosynthesis from carbon dioxide and water. This compound has been used in the
generation of energy in the form of ATP. Blood glucose level monitoring is required to keep
track of sugar (blood glucose) which is significantly important since the main form of
keeping diabetes under the control (Hui et al., 2017). Various diseases associated with high
blood glucose levels (diabetes) can be avoided by early detection of high blood glucose.
Ketone molecules can also be detected in urine tests which occurs with the reduction of
insulin in the blood. Thus, the detection of blood glucose has been found to be significantly
important in the field of medical science (Bebu et al., 2017). Newer methods of blood
glucose level detection play an important role in the initiation of the treatment process for
early diagnosis of a blood associated disease. Spectroscopy, type IV blood tests and finger
prick are the most used tests of blood glucose levels. All these tests are used in order to
determine the concentration of blood glucose which remains in the blood.
Materials and Methods
Materials required
Physical equipment: Test tubes, Beakers, conical flasks, UV-Vis spectrophotometer and
water bath.
Chemical reagents: DNS solution A and B, distilled water and honey

BIOLOGY3
Method used
The assay used to study the concentration of glucose in the blood is known as DNS
(dinitrosalicylic acid). Glucose oxidase determination (GOD) can be used in order to measure
the concentration of glucose in the blood. This process gives a fast and simple estimation of
the concentration of saccharine in blood by measuring the total concentration of reducing
sugars in human blood. In this experiment, this assay will be used to calculate the amount of
glucose present in the honey sample. DNS test is mostly used for the estimation of reducing
sugars by preparing a solution of DNS as a solution A and solution B which consists of
sodium potassium tartrate. Solution A and B are mixed properly to homogenize and complete
the volume to 100 m with distilled water. This solution is stored in an amber bottle at a
temperature of four-degree centigrade. A range of standards is prepared according to the
given table below:
Name of the tube Concentration
Standard tube 1 1.0 ml distilled water
Standard tube 2 0.2 mL glucose solution+0.8 ml distilled
water
Standard tube 3 0.4 mL glucose solution+ 0.6 mL distilled
water
Standard tube 4 0.6 mL glucose solution+ 0.4 mL distilled
water
Standard tube 5 0.8 mL glucose solution+ 0.2 mL distilled
water
Standard tube 6 1.0 mL glucose solution
n.b (1.0 ml) Both tubes kept for both the assays.