Report on Determination of Iron Content in Ferritin
Added on 2023-04-08
6 Pages2104 Words164 Views
PP4003 Report on the Determination of iron content in ferritin Practical
What you need to do:
You should already have written up the practical relating to the “Determination of iron content
in ferritin” in your laboratory workbook. This will be your source information for your final word
processed report and the investigation.
Your report will follow the following format:
Title
Introduction
Materials and Methods
Results (including Tables & Figures)
Discussion
References (in Harvard Format)
Deadlines:
Submission by 9th Jan 2018 for formative feedback
Final assessed submission 5th March 2018
Use the guidelines below to help you complete each section.
1. Introduction
You need to write an introduction with a maximum word count of 500-800. In it you should
describe the experiments you are performing in the context of the question you have been
asked to address.
In this case you are measuring the amount of iron release by the protein ferritin.
You need to briefly give the background of the project, describe the ferritin role in humans and
the diseases associated to an alteration of its level, the ferritin structure, the spectroscopic
technique, and the reactions involved in the determination of the iron, the linear correlation
given by the Beer Law, examples of the absorbance spectroscopy technique applications.
Within the Introduction you should reference at least 3 external sources of information as
evidence of your literature research and these references should be included in a reference
section at the end of the introduction. The referencing should be made in Harvard format as
described in “Cite them Right” which can be accessed via the following link.
http://www.uel.ac.uk/lls/support/harvard/
The references are not included in your word count
2. Materials and Methods
The Materials and Methods should precisely but concisely describe how you performed the
experiments. There is no need to include a list of the materials used. In this section you
need to use full sentences and paragraphs, NOT numbered or bulleted lists. This is a
professional report not a recipe book.
3. Results
The Results should describe the data you collected from these experiments as well as the
meaning of each data (why using Beer law? Explain the logic behind your calculations in the
What you need to do:
You should already have written up the practical relating to the “Determination of iron content
in ferritin” in your laboratory workbook. This will be your source information for your final word
processed report and the investigation.
Your report will follow the following format:
Title
Introduction
Materials and Methods
Results (including Tables & Figures)
Discussion
References (in Harvard Format)
Deadlines:
Submission by 9th Jan 2018 for formative feedback
Final assessed submission 5th March 2018
Use the guidelines below to help you complete each section.
1. Introduction
You need to write an introduction with a maximum word count of 500-800. In it you should
describe the experiments you are performing in the context of the question you have been
asked to address.
In this case you are measuring the amount of iron release by the protein ferritin.
You need to briefly give the background of the project, describe the ferritin role in humans and
the diseases associated to an alteration of its level, the ferritin structure, the spectroscopic
technique, and the reactions involved in the determination of the iron, the linear correlation
given by the Beer Law, examples of the absorbance spectroscopy technique applications.
Within the Introduction you should reference at least 3 external sources of information as
evidence of your literature research and these references should be included in a reference
section at the end of the introduction. The referencing should be made in Harvard format as
described in “Cite them Right” which can be accessed via the following link.
http://www.uel.ac.uk/lls/support/harvard/
The references are not included in your word count
2. Materials and Methods
The Materials and Methods should precisely but concisely describe how you performed the
experiments. There is no need to include a list of the materials used. In this section you
need to use full sentences and paragraphs, NOT numbered or bulleted lists. This is a
professional report not a recipe book.
3. Results
The Results should describe the data you collected from these experiments as well as the
meaning of each data (why using Beer law? Explain the logic behind your calculations in the
determination of the iron value in ferritin).
Mean and standard deviation of your data MUST be reported.
Graphs, tables and the results of any statistical analysis should be included in this section.
We suggest you show
A table with the absorbance values of your standard solutions with the corresponding
calculated iron Fe(II) concentrations.
A graph with your standard curve (including the line equation and R2 value).
A table with the absorbance values of the solutions of ferritin with the corresponding
concentration calculation
Each figure or table should also have a corresponding legend. The Figures and Figure legends
are not included in the word count
Report the calculations for the determination of the moles of iron per mL of solution.
4. Discussion
The Discussion should present a short explanation of your results and what they mean in
terms of the question that was being addressed.
You need to discuss the validity of your methodology. If there were any experimental problems
that might make you question the validity of your results these should also be discussed. You
can refer back to references used in the Introduction or add new ones to support statements
made in the Discussion.
The small print
You should aim for 1200-1500 words with an absolute maximum word count of 1600 for the
entire report. This does not include References and Figure legends.
A draft of the report should be submitted through Turn It In on the PP4003 Moodle site by 9th
Jan 2018 for you to receive formative feedback. There will be a separate Turn It In link for
each practical group. It is in your own interest to submit a draft by 9th Jan 2017 for feedback
as this will help you improve your final mark.
The final submission the report should be submitted through Turn It In on the BS4003 Moodle
site by 5th March 2018 this should give you time to incorporate feedback. There will be a
separate Turn It In link for each practical group. The mark will contribute 30% to your final
module grade.
Citations and References
Cutting and pasting text from the internet or copying text from books is plagiarism, an
academic offence that will result in a severe punishment. You must paraphrase, i.e. write
information in your own words and cite the source of the information by referencing.
Mean and standard deviation of your data MUST be reported.
Graphs, tables and the results of any statistical analysis should be included in this section.
We suggest you show
A table with the absorbance values of your standard solutions with the corresponding
calculated iron Fe(II) concentrations.
A graph with your standard curve (including the line equation and R2 value).
A table with the absorbance values of the solutions of ferritin with the corresponding
concentration calculation
Each figure or table should also have a corresponding legend. The Figures and Figure legends
are not included in the word count
Report the calculations for the determination of the moles of iron per mL of solution.
4. Discussion
The Discussion should present a short explanation of your results and what they mean in
terms of the question that was being addressed.
You need to discuss the validity of your methodology. If there were any experimental problems
that might make you question the validity of your results these should also be discussed. You
can refer back to references used in the Introduction or add new ones to support statements
made in the Discussion.
The small print
You should aim for 1200-1500 words with an absolute maximum word count of 1600 for the
entire report. This does not include References and Figure legends.
A draft of the report should be submitted through Turn It In on the PP4003 Moodle site by 9th
Jan 2018 for you to receive formative feedback. There will be a separate Turn It In link for
each practical group. It is in your own interest to submit a draft by 9th Jan 2017 for feedback
as this will help you improve your final mark.
The final submission the report should be submitted through Turn It In on the BS4003 Moodle
site by 5th March 2018 this should give you time to incorporate feedback. There will be a
separate Turn It In link for each practical group. The mark will contribute 30% to your final
module grade.
Citations and References
Cutting and pasting text from the internet or copying text from books is plagiarism, an
academic offence that will result in a severe punishment. You must paraphrase, i.e. write
information in your own words and cite the source of the information by referencing.
A more detailed description of how a scientific report should be written is given
below. These guidelines were adapted by Dr. Guiliano from “The Structure of Scientific
Reports” available at:
http://unilearning.uow.edu.au/report/2b.html
This online resource gives a good description of how scientific reports should be written and I
would suggest you read it in full.
1. Introduction
In your introduction, you need to let the readers (or markers) of your report know why the report
is important and what exactly the report is about. It is essential to establish these things
because it places the reader / marker in a better position to understand the significance of the
material presented in the rest of the report. In your introduction, you need to answer questions
such as
What do you hope to learn from the experiment?
What question is being asked?
Why is this research important?
The introduction starts generally by introducing the broad context within which your research
fits. You need to provide a short review of the topic and how it impacts on your research
question. The literature needs to provide the reader / marker of your report with an
understanding of the conceptual and theoretical background, context and justification for the
experiments you are undertaking.
Your literature review uses both the past tense and the present tense. The past tense is used
to refer to a particular experiment and the specific results of a particular experiment that has
been carried out in the past. The present tense is used to refer to information that is not
confined to a particular experiment.
For example,
PAST tense: Reijenga (2013) studied the technique in ..., The model predicted that .....
PRESENT tense: Table 1 shows that.....
The introduction ends with a statement of your specific hypothesis or hypotheses. This
statement of the hypothesis should logically follow on from your literature review and you may
want to make an explicit link between the variables you are manipulating or measuring in your
study and previous research.
2. Materials and Methods
The purpose of this section is to precisely describe the method and materials used to conduct
your experiment with enough detail so someone else could repeat the same procedure. In the
method section of the report you should use the past tense since you are describing what you
did. For example:
• A dilution series was performed....
• The absorbance was read at...
Furthermore, as the focus in this section is on what was done rather than who did it, the passive
below. These guidelines were adapted by Dr. Guiliano from “The Structure of Scientific
Reports” available at:
http://unilearning.uow.edu.au/report/2b.html
This online resource gives a good description of how scientific reports should be written and I
would suggest you read it in full.
1. Introduction
In your introduction, you need to let the readers (or markers) of your report know why the report
is important and what exactly the report is about. It is essential to establish these things
because it places the reader / marker in a better position to understand the significance of the
material presented in the rest of the report. In your introduction, you need to answer questions
such as
What do you hope to learn from the experiment?
What question is being asked?
Why is this research important?
The introduction starts generally by introducing the broad context within which your research
fits. You need to provide a short review of the topic and how it impacts on your research
question. The literature needs to provide the reader / marker of your report with an
understanding of the conceptual and theoretical background, context and justification for the
experiments you are undertaking.
Your literature review uses both the past tense and the present tense. The past tense is used
to refer to a particular experiment and the specific results of a particular experiment that has
been carried out in the past. The present tense is used to refer to information that is not
confined to a particular experiment.
For example,
PAST tense: Reijenga (2013) studied the technique in ..., The model predicted that .....
PRESENT tense: Table 1 shows that.....
The introduction ends with a statement of your specific hypothesis or hypotheses. This
statement of the hypothesis should logically follow on from your literature review and you may
want to make an explicit link between the variables you are manipulating or measuring in your
study and previous research.
2. Materials and Methods
The purpose of this section is to precisely describe the method and materials used to conduct
your experiment with enough detail so someone else could repeat the same procedure. In the
method section of the report you should use the past tense since you are describing what you
did. For example:
• A dilution series was performed....
• The absorbance was read at...
Furthermore, as the focus in this section is on what was done rather than who did it, the passive
End of preview
Want to access all the pages? Upload your documents or become a member.
Related Documents
Generate a Standard Curvelg...
|9
|1583
|88
SLE132 Prac 4 Part 2: Photosynthesis Report Templatelg...
|5
|830
|141
VEN1102 Engineering Physics Assignmentlg...
|6
|1368
|349
Writing up your project: Practical advicelg...
|1
|630
|106
Wastewater Engineering Laboratory Reportlg...
|2
|683
|244
Computer Security Assignmentlg...
|12
|4299
|162