This study analyzes the effect of reaction time on gender using one-way ANOVA and t-test analysis. The study examines the difference between the reaction time of congruent, neutral, and incongruent on gender. The data is collected for 113 females and 17 males for each type of reaction time. The study finds significant differences between the mean reaction time of congruent and neutral, congruent and incongruent, and neutral and incongruent.