THE PROTEIN DISCOVERY AND ANALYSIS

Running head: PROTEIN DISCOVERY AND ANALYSIS
BOVINECHYMOSIN
Name of the Student
Name of the University
Author Note

PROTEIN DISCOVERY AND ANALYSIS1
Table of Contents
Introduction................................................................................................................................2
The primary structure of PET protein........................................................................................2
The physico-chemical properties of PET protein.......................................................................3
Initial purification of the protein................................................................................................5
Present purification of the protein..............................................................................................7
Bibliography...............................................................................................................................9

PROTEIN DISCOVERY AND ANALYSIS2
Introduction
Bovine chymosin is an aspartyl protease extracted protein obtained from abomasum of
suckling calves. This protein is now synthesised in vivo as preprochymosin and has been
found to be secreted as prochymosin that is auto catalytically activated to form chymosin.
Chymosin has been found to be engineered inside laboratories and the gene encoding
chymosin has been cloned and expressed in bacteria and yeast also. This paper will discuss
the structure, physico-chemical properties and purification processes of bovine chymosin in
brief.
The primary structure of PET protein
10 20 30 40 50
MRCLVVLLAV FALSQGAEIT RIPLYKGKSL RKALKEHGLL EDFLQKQQYG
60 70 80 90 100
ISSKYSGFGE VASVPLTNYL DSQYFGKIYL GTPPQEFTVL FDTGSSDFWV
110 120 130 140 150
PSIYCKSNAC KNHQRFDPRK SSTFQNLGKP LSIHYGTGSM QGILGYDTVT
160 170 180 190 200
VSNIVDIQQT VGLSTQEPGD VFTYAEFDGI LGMAYPSLAS EYSIPVFDNM
210 220 230 240 250
MNRHLVAQDL FSVYMDRNGQ ESMLTLGAID PSYYTGSLHW VPVTVQQYWQ
260 270 280 290 300
FTVDSVTISG VVVACEGGCQ AILDTGTSKL VGPSSDILNI QQAIGATQNQ
310 320 330 340 350
YGEFDIDCDN LSYMPTVVFE INGKMYPLTP SAYTSQDQGF CTSGFQSENH
360 370 380
SQKWILGDVF IREYYSVFDR ANNLVGLAKA I
Features Positions with colours
Signal peptide 1-16, Red
Propeptide 17-58, Yellow
Chain 59- 381, Green
Disulphide bond 105-110, Pink

PROTEIN DISCOVERY AND ANALYSIS3
Disulphide bond 265-269, Pink
Disulphide bond 308-341, Pink
The physico-chemical properties of PET protein
Mature peptide sequence:
>sp|P00794|CHYM_BOVIN Chymosin OS=Bos taurus OX=9913 GN=CYM PE=1 SV=3
LLAVFALSQGAEITRIPLYKGKSLRKALKEHGLLEDFLQKQQYGISSKYSGFGE
VASVPLTNYLDSQYFGKIYLGTPPQEFTVLFDTGSSDFWVPSIYCKSNACKNHQRFDPRK
SSTFQNLGKPLSIHYGTGSMQGILGYDTVTVSNIVDIQQTVGLSTQEPGDVFTYAEFDGI
LGMAYPSLASEYSIPVFDNMMNRHLVAQDLFSVYMDRNGQESMLTLGAIDPSYYTGSLHW
VPVTVQQYWQFTVDSVTISGVVVACEGGCQAILDTGTSKLVGPSSDILNIQQAIGATQNQ
YGEFDIDCDNLSYMPTVVFEINGKMYPLTPSAYTSQDQGFCTSGFQSENHSQKWILGDVF
IREYYSVFDRANNLVGLAKAI
Physico-chemical properties:
User-provided sequence:
10 20 30 40 50 60
LLAVFALSQG AEITRIPLYK GKSLRKALKE HGLLEDFLQK QQYGISSKYS GFGEVASVPL
70 80 90 100 110 120
TNYLDSQYFG KIYLGTPPQE FTVLFDTGSS DFWVPSIYCK SNACKNHQRF DPRKSSTFQN
130 140 150 160 170 180
LGKPLSIHYG TGSMQGILGY DTVTVSNIVD IQQTVGLSTQ EPGDVFTYAE FDGILGMAYP
190 200 210 220 230 240
SLASEYSIPV FDNMMNRHLV AQDLFSVYMD RNGQESMLTL GAIDPSYYTG SLHWVPVTVQ
250 260 270 280 290 300
QYWQFTVDSV TISGVVVACE GGCQAILDTG TSKLVGPSSD ILNIQQAIGA TQNQYGEFDI
310 320 330 340 350 360
DCDNLSYMPT VVFEINGKMY PLTPSAYTSQ DQGFCTSGFQ SENHSQKWIL GDVFIREYYS
370
VFDRANNLVG LAKAI
Number of amino acids: 375
Molecular weight: 41477.73
Theoretical pI: 4.82
Amino acid composition:
Ala (A) 19 5.1%
Arg (R) 8 2.1%
Asn (N) 15 4.0%